FGRB

 

 

FGRB

50x (formaldehyde gel-running buffer), p=1.104g/ml (store at +4oC):

Conc.Stock0.5L1.0L2.0L
MOPS (pH7.0)1.0M209.27g/M104.64g209.27g418.54g
AcONax3H2O0.5M136.08g/M34.02g68.04g136.08g
EDTAx2H2O50mM372.3g/M9.31g18.62g37.23g
H2O mQ404.0ml808ml1616ml
Conc.Stock0.5L1.0L2.0L
MOPS (pH7.0)1.0M209.27g/M104.64g209.27g418.54g
AcONa anhydrous0.5M82.04g/M20.51g41.02g82.04g
EDTA x 2H2O50mM372.3g/M9.31g18.62g37.23g
H2O mQ417.5ml835ml1670ml
  • adjust pH7.0 10N NaOH (per 500ml of buffer ~22ml), filter-sterilize through 0.22┬Ám filter;
  • solution becomes yellow during the storage on the light, but it has no effect on the solution quality.