PBS Solutions

 

 

PBS

pH(1x)=7.4, (store at NT or at 4oC):

1xConc.Stock100ml500ml
KH2PO41.7mM17mM136.1g/M0.231g1.157g
Na2HPO45.2mM52mM142g/M0.738g3.692g
NaCl150mM1.5M58.44g/M8.77g43.83g
H2O mQ    
1xConc.Stock100ml500ml
KH2PO41.7mM17mM136.1g/M0.231g1.157g
Na2HPO4x7H2O5.2mM52mM268.1g/M1.394g6.971g
NaCl150mM1.5M58.44g/M8.77g43.83g
H2O mQ    
  • autoclave sterilization;
  • pH is concentration dependent; thus check pH only for diluted 1x buffer;
  • pH of 10x should be ~6.8 (adjust by NaOH if necessary).