SSPE Solutions

 

 

SSPE

pH 7.4

Conc.Stock1L
NaCl3M58.44g/M175g
NaH2PO4xH2O200mM137.99g/M27.6g
EDTA20mM372.24g/M7.4g
  • adjust pH by 10N NaOH.
  • RNase A (DNase free)

    (store at -20oC, working quantities may be stored at 4oC)

    1. solubilize 10mg/ml in 1-50mM AcONa (pH4.5);
    2. 15' in boiling water bath, then cool slowly to NT;