TAE

 

 

TAE

1x pH=7.6:

Tris-acetate 40mM
EDTA 1mM

(store the large stock at 4oC, working quantity (~100ml) at NT)

Conc.Stock100ml150ml200ml250ml
Tris-base2M121.1g/M24.22g36.33g48.44g60.55g
EDTA0.05M372.3g/M1.862g2.792g3.723g4.654g
Acetic acid~1M17.4M ~5.71ml
~6.00g
~8.57ml
~9.00g
~11.42ml
~12.00g
~14.28ml
~15.00g

H2O mQ     

p(50x)=1.0878

  • it is convenient to solubilize Tris and EDTA first, and only then add acid to adjust pH.