TBE

 

 

TBE

10x, pH ~8.3;(store at NT in clean bottle)

1x10xStock1L
Tris base89mM0.89M121.14g/M107.8g
Boric acid89mM0.89M61.83g/M55.03g
EDTA, pH 8.02mM20mM500mM40ml
43.84g

H2O  mQ863.3ml
  • 10x stock solution is stable at NT if to clean it through 0.4┬Ám filter;
  • for PAAG it is used as 1x, for agarose gel - as 0.5x;
  • for agarose gels it is used for separation of small (< 200bp) fragments. Generally accepted, that the purification of fragments is more complex from TBE gels.