TGB

 

 

TGB

Tris-Glycine buffer 5x, pH8.3, (store the main stock at +4oC, working quantity at NT).

1xConc.Stock1L1.5L
Tris-base25mM125mM121.1g/M15.1g22.71g
Glycine250mM1.25M75.05g/M94g141g
SDS0.1%0.5%10%50ml75ml
H2O  mQ   

Loading buffer

10x, (store at NT).

Conc.Stock1ml5ml10ml25ml
Xilene cyanol0.1%solid1.0mg5.0mg10mg25.0mg
Bromph. blue0.1%solid1.0mg5.0mg10mg25.0mg
SDS0.5%10%50µl250µl500µl1.25ml
EDTA, pH8.00.1M0.5M0.2ml1.0ml2.0ml5.0ml
Glycerol50%100% 0.5ml
0.625g
2.5ml
3.125g
5.0ml
6.25g
12.5ml
15.63g

H2O mQ0.25ml1.25ml2.5ml6.25ml