Neutralization buffer

 

 

Neutralization buffer

(store at NT).

Conc.Stock150ml300ml500ml750ml
Tris Cl, pH7.41M121.1g/M18.15g36.3g60.5g90.75g
NaCl1.5M58.44g/M13.15g26.30g43.83g65.75g
HCl~0.81M11.6M/L~10.5ml~21ml~35ml~52.5ml
EDTA (optional)1mM0.5M0.3ml0.6ml1ml1.5ml