Restriction buffers 10x

 

 

Restriction buffers 10x

À.

Conc.Stock100µl1ml
Tris Ac (pH7.9 37oC)33mM1M33µl330µl
MgAc10mM1M10µl100µl
KAc66mM5M13.2µl132µl
DTT0.5mM1M0.5µl5µl
H2O mQ43.3µl433µl

B.

Conc.Stock100µl1ml
Tris Cl (pH 8.0 37oC))10mM1M10µl100µl
MgCl25mM1M5µl50µl
NaCl100mM5M20µl200µl
b-MeEtOH1mM1M1µl10µl
H2O mQ64µl640µl

L.

Conc.Stock100µl1ml
Tris Cl (pH 7.5 37oC)10mM1M10µl100µl
MgCl210mM1M10µl100µl
DTT1mM1M1µl10µl
H2O mQ79µl790µl

M.

Conc.Stock100µl1ml
Tris Cl (pH7.5 37oC)10mM1M10µl100µl
NaCl50mM5M10µl100µl
MgCl210mM1M10µl100µl
DTT1mM1M1µl10µl
H2O mQ69µl690µl

H.

Conc.Stock100µl1ml
Tris Cl (pH7.5 37oC)50mM1M50µl50µl
MgCl210mM1M10µl100µl
NaCl100mM5M20µl200µl
DTT1mM1M1µl10µl
H20 mQ19µl190µl