DTT Calculator

 

 

DTT

C4H10O2S2 Mw=154.2g/M; (store at -20oC).

Conc.Stock%(w/w)1ml5ml10ml20ml
DTT154.2g/M1M14.550.155g0.773g1.55g3.09g
H2O mQ85.45905 ┬Ál4.53ml9.05ml18ml

p~1.06g/ml

Conc.Stock1ml5ml10ml20ml
DTT154.2g/M2.2M0.339g1.696g3.394g6.787g
H2O mQ     
  • filter sterilization;
  • no DEPC treatment;
  • we do not know why, but many manuals (probably, after "Maniatis") recommend to prepare it in 10mM AcNa, pH 5.2.