ATP Calculator

C10H14N5O13P3Na2

 

 

ATP

C10H14N5O13P3Na2 Mw=551.1 g/M; (store at -20oC).

Conc.Stock1ml
ATP0.1M551.1g/M55.1mg
H2O mQ  

Untested information 0.1M ATP (pH 7.2): 5.71% (w/w) ATP, 84.86% (w/w) H2O, 9.43% (w/w) 2M Tris base.

  • adjust to pH7.5 by 2M NaOH (~70-80µl);
  • prepare about 700µl, dilute 2000 times (it is the final dilution in the spectrofotometric cell), check the optical density:
  • c[M]=A259/15.4x103=A259 1:2000x0.130; then, adjust the final volume.