Phenol

C6H5OH

 

 

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* For RNA extraction it is better to saturate (just add) "acidic phenol" by the following buffer: (store at 4oC):

Conc.Stock100ml
AcONa, pH 5.150mM3M1.67ml
EDTA10mM0.5M2.0ml
H2O mQ96.0ml
  • check pH and adjust by diluted (1:100) acetic acid if necessary.
  • Untested information there was note in the "Mitchell Group Methods" that acidic phenol is much more stable, then the neutral. They recommend preparing neutral phenol in small quantities only for a few weeks.