TrisCL Calculator

C12H22O11

 

 

Tris Cl

C4H11O3N.
Conc.Stock%(w/w)50ml100ml250ml
Sucrose1M342.30g/M30.33017.115g34.23g85.58g
H2O mQ69.67039.315ml78.63ml196.58ml

Tris Cl

C4H11O3N; Mw=121.1g/M; (store at 4oC).

Conc.Stock50 ml100 ml150ml200ml
Tris-base1M121.1g/M6.0612.11g18.17g24.22g
H2O to the final weight mQ52.03g104.06g156.09g208.12g

1M: p=1.0406

Untested information 2M Tris base: 22.90%(w/w) Tris base, 77.10%(w/w) H2O; p=1.058

  • not to treat by DEPC;
  • autoclave sterilization;
  • pH of Tris-buffers is dependent from concentration. If to take as original the 50mM solution:
  • pH(500mM) => + 0.05
    pH(5mM) => - 0.05

  • pH drops on 0.028 when the temperature rise on 1oC.
  • Temperature dependence of pH for Tris Cl 50mM.

    pH at g/50ml 1M or
    g/liter for 0.05 M
    5째C25째C37째C Tris HClTris BaseH2O Tris HClTris BaseH2O
    7.557.006.70 7.280.4744.28
    7.667.106.80 7.130.5744.33
    7.767.206.91 7.020.6744.34
    7.897.307.02 6.850.8044.38
    7.977.407.12 6.610.9744.45
    8.077.507.22 6.351.1844.50
    8.187.607.30 6.061.3944.58
    8.267.707.40 5.721.6644.65
    8.377.807.52 5.321.9744.74
    8.487.907.62 4.882.3044.85
    8.588.007.71 4.442.6544.94
    8.688.107.80 4.022.9745.04
    8.788.207.91 3.543.3445.15
    8.888.308.01 3.073.7045.26
    8.988.408.10 2.644.0345.36
    9.098.508.22 2.214.3645.46
    9.188.608.31 1.834.6545.55
    9.288.708.42 1.504.9045.63
    9.368.808.51 1.235.1345.67
    9.478.908.62 0.965.3245.75
    9.569.008.70 0.765.4745.80
    9.679.108.79 0.695.5345.81

    TrisCl: 250ml 1M

    V HClV HClpH
     010.44
     19.54
     29.26
     39.08
     3.78.98
    5ml 8.76
    6ml 8.67
     108.4
    11ml 8.22
     148.13
    13.5ml 8.06
    14ml 8.04
    15ml 7.97
     177.92
    16.5ml 7.82
    16.7ml 7.8
    17ml 7.77
    18.3ml 7.63
    18.6ml 7.59
     207.75
     237.4