SOC,SOB

 

 

SOC, SOB

(store at NT).

Stock200ml400ml
Bactotriptone 4.0g8.0g
Bacto Yeast extr. 1.1g2.2g
NaCl5M0.4ml0.8ml
KCl1M2.0ml4ml
H2O ~196ml~391ml
  1. after autoclaving to cool to <60oC;
  2. add
  3. 0.01V 2M Mg2+{= 1M MgCl2 + 1M MgSO4}  
    0.01V 2M glucose;  

  4. 0.2┬Ám filter sterilization;
  5. store at -20oC, (-70oC) in small aliquots.

SOB - the same solution without glucose.