LB, LTB Calculator

 

 

LB, LTB

 Stock100ml150ml200ml300ml600ml750ml
1.Bactotriptonesolid1g1.5g2.0g3.0g6.0g7.5g
2.Bacto Yeast extr.solid0.5g0.75g1.0g1.5g3.0g3.75g
3.NaClsolid1.0g1.5g2.0g3.0g6.0g7.5g
4.H2OmQ95.3g143g190.7g286g572g715g
5.agar topsolid0.65g0.98g1.3g1.95g3.90g4.88g
6.agar bot.solid1.5g2.3g3.0g4.5g9.0g11.3g
7.NaOH10N16.7µl25µl33.4µl50µl100µl125µl
8.MgSO41M1ml1.5ml2.0ml3.0ml6.0ml7.5ml

LTB: p.1, 3, 4, 7, 8.
LB: p.1, 2, 3, 4, 7.
LB/Mg: p.1, 2, 3, 4, 7, 8.


LB (low salt)

For medium with Zeocine pH 7.0

Stock100ml150ml200ml300ml600ml750ml
Bactotriptonesolid1g1.5g2.0g3.0g6.0g7.5g
Bacto Yeast extr.solid0.5g0.75g1.0g1.5g3.,0g3.75g
NaClsolid0.5g0.75g1.0g1.5g3.,0g3.75g
H2OmQ95.3g143g190.7g286g572g715g
NaOH10N16.7µl25µl33.4µl50µl100µl125µl