Terrific broth

 

 

Terrific Broth

pH 7.0

100ml150ml200ml300ml667ml
Bactotriptone1.2g1.8g2.4g3.6g8.0g
Bacto Yeast extr.2.4g3.6g4.8g7.2g16.0g
Glycerol0.4ml
0.504g
0.6ml
0.757g
0.8ml
1.009g
1.2ml
1.513g
2.667ml
3.362g

H2O to V90ml135ml180ml270ml600ml
add after autoclaving:
KPO4, pH7.0 1.0M10ml15ml20ml30ml66.7ml